Abstract:

Indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of V. parahaemolyticus . Specific polyclonal antibody that produced in New Zealand white rabbits were used in the indirect competitive ELISA.The best reaction concentration of antigen and antibody is 107 CFU/ml and 1: 4,000, respectively, and ethe most effective concentration of the secondary HRP-labeled antibody is 1: 1,000. This standardized system was established for detecting V. parahaemolyticus in artificially infected seafood and actual seafood respectively. The detection limit is 104 CFU/ml, detection time is 8hours; however, the accuracy could be improved to 103 CFU/ml after pre-enrichment for 8hours. This system could be of practical application since it was close to the detection result by traditional detection methods.

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Date 2009/04/15

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