Abstract:

Hyaluronic acid (HA) is commonly extracted from terrestrial and bacterial sources. However, due to the risk associated with animal and bacterial derived contaminants and the laborious production that tend to compromise the quality of HA, research studies have recently shifted to the exploration of HA in marine resources and the enhancement of the protocols for its production. In this study, isolation of HA from yellowfin tuna Thunnus albacares (Bonnaterre, 1788) eyeball was carried out through standardisation of the different steps in HA isolation, including tissue extraction, enzymatic hydrolysis, precipitation with cetylpiridinium chloride (CPC) - sodium chloride (NaCl) solution, filtration and diafiltration, precipitation in alkaline hydroalcoholic solution, and lyophilisation. Results revealed that the highest HA yield and purity were observed in the indirect treatment wherein the raw material was pre-treated with acetone, formaldehyde, and sodium acetate solution prior to water extraction and incubation of the mixture for 24 h. Enzymatic hydrolysis revealed that 6 h was the maximum tissue hydrolysis period. Results further showed that the optimum conditions for HA isolation were through the use of 3 % CPC:3M NaCl concentration for recovery and fractionation and a 1:3 mL.mL^-1 supernatant:ethanol ratio for alcoholic precipitation. The data gathered from this study could help maximise the benefits of the tuna processing waste in the country and may open a new opportunity for more cost-effective production of a valuable bioactive compound from a natural source.

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Date 2020/03/31

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