Abstract:

Sperm of Mekong giant catfish (Pangasius gigas; formerly Pangasianodon gigas) were cryorpeserved in 5 or 9% dimethyl sulfoxide (DMSO) in bicarbonate buffer (BCB) or calcium-free Hank’s balanced salt solution (C-F HBSS) at different cooling rates (-5, -12, -22 or -120 ºC•min^-1) and stored in liquid nitrogen for 3-4 months. Pooled ova of three female Gunther’s walking catfish (Clarias macrocephalus) were inseminated with cryopreserved semen of Mekong giant catfish and fresh semen of the walking catfish (control). There was no significant difference in fertility of sperm cryopreserved in the two extenders. Best results were obtained when sperm were cryopreserved in 9% DMSO in either extender in 5 ml cryotubes and frozen at -12 ºC•min^-1. The percentage of fertilization was 65-66% (actual) and 73-74% (control).

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Date 1995/12/01

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